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KMID : 0378019800230070073
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New Medical Journal
1980 Volume.23 No. 7 p.73 ~ p.77
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Biological characteristics of Hemophilus vaginalis
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鞠允鎬/Kook, Y.H.
李光浩/張友鉉/Lee, K.H./Chang, W.H.
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Abstract
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23 local strains of H. vaginalis isolated from the vagina of the women who visited Family Health Clinic, The Institute of Reproductive Medicine and Population, College of Medicine Seoul National University, and the V.D. clinic of Health center, Inchon, were tested to clarify their biological characters and then to suggest a proper identifying protocol.
2 vaginal swabs were obtained from each woman. One swab was used for wet smear to find clue cells, and for gram staining to find gram negative rods on epithelial cells, and the other for further identification of microorganism.
(1) Morphology and staining characters
All strains showed uniform pinpoint sized colonies with convex, entire margin. Hemolytic patterns were a though some strains showed no hemolysis. They were gram negative coccobacilli containing metachromatic and lipid granules, and lying end to end and angled arrangement.
(2) Biochemical characters
All strains were inhibited by 3% H2O2a and catalase negative. Tellurite reduction (0.01%), indol production, V-P reaction, urease test, nitrate reduction were negative, and 10 strains among 23 strains grew in KCN medium. Though there were some variations in the ability of sugar fermentation, most of the tested strains could produce acid from dextrose, dextrin, maltose, fructose, and galactose, but no acid from lactose, and mannitol.
(3) Growth conditions
Optimal growth conditions for H. vaginalis were 37C, and pH 7.07.5. There was no difference in growth between aerobic and anaerobic conditions, but growth was enhanced under 5% CO2. They didn´t require X or V factor.
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